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S5133

Sigma-Aldrich

Schiff′s fuchsin-sulfite reagent

liquid

Synonym(s):

Schiff reagent

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About This Item

Empirical Formula (Hill Notation):
C20H21N3O3S
Molecular Weight:
383.46
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Schiff′s fuchsin-sulfite reagent, suitable for detection of glycoproteins

Assay

3-5 mM iodine (I) basis (titration)

Quality Level

100

form

liquid

color

colorless

solubility

water: soluble

density

0.990 g/cm3

suitability

suitable for detection of glycoproteins

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Related Categories

General description

Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples.

Application

Schiff′s fuchsin-sulfite reagent has been used:

  • in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins
  • to stain ovaries and testis
  • to stain tomato and Medicago primary roots
  • to stain undifferentiated sarcomas (USARCs)
It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes.

Preparation Note

Prepared using pararosaniline.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H350

Hazard Classifications

Carc. 1B - Met. Corr. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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