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S0751

Sigma-Aldrich

Sodium phosphate monobasic

ReagentPlus®, ≥99.0%

Synonym(s):

Monosodium dihydrogen orthophosphate, Monosodium phosphate, Sodium dihydrogen phosphate

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About This Item

Linear Formula:
NaH2PO4
CAS Number:
7558-80-7
Molecular Weight:
119.98
EC Number:
231-449-2
MDL number:
MFCD00003527
UNSPSC Code:
12161700
eCl@ss:
38070208
PubChem Substance ID:
24899443
NACRES:
NA.21
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Quality Level

200

product line

ReagentPlus®

Assay

≥99.0%

form

powder or crystals

technique(s)

DNA extraction: suitable
electrophoresis: suitable
immunocytochemistry: suitable

pH

4.0-4.5 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: 100 mg/mL, clear, colorless

application(s)

detection

SMILES string

[Na+].OP(O)([O-])=O

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General description

Sodium phosphate monobasic acts as an acidulant in laxatives and effervescent powders. It is used in beverages, cheese and soft drink formulations.[1]

Application

Sodium phosphate monobasic has been used:
  • as a reagent for virus transfection and fiber implantation in mouse[2]
  • in phosphate buffer for immunocytochemistry[3] and to detect individual catalase activity by PAGE (polyacrylamide gel electrophoresis)[4]
  • as a buffer for cycling assay[5]
  • in automated DNA extraction method[6]
  • as a chemical in artificial cerebro-spinal fluid (aCSF) solution for the preparation of brainstem-spinal cord from rats[7]

Other Notes

Go to Redi-Dri™ Product Listing RDD007

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    WXBF5481V
    0000411031
    0000411031
    0000411033
    0000411034

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    In vitro Brainstem-spinal Cord Preparation from Newborn Rat
    Le Gal JP, et al.
    The Journal of Neuroscience (2016)
    Dictionary of Food ingredients (2001)
    Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry
    Kanamori KS, et al.
    Bio-protocol, 8(14) (2018)
    Automated and manual methods of DNA extraction for Aspergillus fumigatus and Rhizopus oryzae analyzed by quantitative real-time PCR
    Francesconi A, et al.
    Journal of Clinical Microbiology, 46(6), 1978-1984 (2008)
    Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE) in Cell Extracts from Pseudomonas aeruginosa
    Pezzoni M, et al.
    Microbiology (2016)
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