Skip to Content
Merck
CN
All Photos(3)

Documents

Safety Information

PPB022

Sigma-Aldrich

Tris buffer

Pre-set, pH 7.0, pHast Pack™, powder

Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
0.1M Tris
UNSPSC Code:
12161700
NACRES:
NA.25

Quality Level

100

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.0

solubility

soluble, clear, colorless

cation traces

Fe: ≤10 ppm

foreign activity

DNase, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

General description

Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Application

Tris buffer is used in:
  • Western blot
  • Protein gel electrophoresis
  • cell culture (sterile filter buffer)
  • As a buffer component

Features and Benefits

  • Fast, ready-to-use buffer
  • No need to measure and adjust the pH
  • Saves time and minimizes effort in the lab
  • Biological tests: free of DNase, RNase, Protease, and Nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 1

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service