PPB019
Bis-Tris Bicine EDTA buffer
pH 7.2, pHast Pack™, powder
Synonym(s):
1X Bis-Tris transfer buffer, Bis-Tris
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105319
Product Name
Bis-Tris Bicine EDTA buffer, pH 7.2, pHast Pack™, powder
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry)
pH
7.2
cation traces
Pb: ≤5 ppm
foreign activity
DNAse, free
NICKase, free
Protease, free
RNAse, free
storage temp.
room temp
Quality Level
Related Categories
Application
Bis-Tris buffer may be used in western blot as a transfer buffer.
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjustment needed
- Certified quality for reproducibility
- Minimizes exposure to toxic chemicals
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm
General description
Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.
Other Notes
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Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
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Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
Regulatory Information
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