Skip to Content
Merck
CN

PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack™, powder

Synonym(s):

1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Tris-Glycine Buffer, pH 8.3, pHast Pack™, powder

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white to off-white

pH

8.3

solubility

soluble, clear, colorless to faintly yellow

cation traces

Fe: <10 ppm

foreign activity

DNase, none detected
Endonuclease
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected

storage temp.

room temp

Quality Level

100

Related Categories

Application

Suitable for use as:
  • Transfer buffer component in western blot
  • Running buffer in native gel electrophoresis for protein separation
  • Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Features and Benefits

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Saves time and minimizes the effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

General description

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCK3077

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service