PPB015
Tris-Glycine Buffer
pH 8.3, pHast Pack™, powder
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Synonym(s):
1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
product line
pHast Pack™
Quality Level
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white to off-white
pH
8.3
solubility
soluble, clear, colorless to faintly yellow
cation traces
Fe: <10 ppm
foreign activity
DNase, none detected
Endonuclease
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected
storage temp.
room temp
General description
Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.
Application
Suitable for use as:
- Transfer buffer component in western blot
- Running buffer in native gel electrophoresis for protein separation
- Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Saves time and minimizes the effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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