Tris Acetate-EDTA buffer

Tris Acetate-EDTA (TAE) buffer, with a pH range of 8.1 – 8.4, is typically used for agarose gel electrophoresis, especially for the recovery of DNA. For larger double-stranded DNA fragments (>12kB) TAE buffer is preferred. However, the buffering capacity can become exhausted if electrophoresis time is extended. Buffer circulation or replacement during electrophoresis can remedy the lower buffering capacity.

Suitable as a running and gel preparation buffer in:

• DNA agarose gel electrophoresis
• Non-denaturing RNA agarose gel electrophoresis for RNA >1500 bases
• Denaturing gradient gel electrophoresis
• Northern and Southern blotting

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Foil pouches

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 40mM Tris-Acetate, 1mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis.

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

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