Skip to Content
Merck
CN
All Photos(2)

Documents

PPB005

Sigma-Aldrich

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20, pHast Pack™

Synonym(s):

PBS + 0.05% TWEEN® 20, PBS with 0.05% Tween 20, PBS-T, PBST

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12161700
NACRES:
NA.21

product name

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4, pHast Pack™, powder

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.2—7.6 (25 °C)

solubility

soluble, clear to hazy, colorless

cation traces

Fe: <10 ppm

foreign activity

DNase, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Phosphate Buffered Saline with 0.05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Application

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.

Features and Benefits

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Save time and minimize effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na2HPO4, 1.8 mM KH2PO4, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysis
N'Guessan et al.
iScience, 26, 107394-107394 (2023)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service