Skip to Content
Merck
CN

PHR1428

HEPES

certified reference material,pharmaceutical secondary standard

Synonym(s):

HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Sign In to View Organizational & Contract Pricing

Select a Size

About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
230-907-9
MDL number:
MFCD00006158
UNSPSC Code:
41116107
PubChem Substance ID:
329822594
NACRES:
NA.24

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

HEPES, Pharmaceutical Secondary Standard; Certified Reference Material

grade

certified reference material
pharmaceutical secondary standard

Quality Level

300

API family

hepes

CofA

current certificate can be downloaded

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-30°C

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2.


Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Application

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
  • HEPES has been used in a wide variety of applications, including tissue culture.
  • It is used to buffer cell culture media in air.
  • Finds its usage in invitro experiments on Mg.
  • Recognized as one of Dr.Good′s recommended buffers, used in some cell culture media as a buffering agent and also in various biochemical reactions.
  • Recently, in the production of radiopharmaceuticals, it is the buffer of choice for scientific labeling.

HEPES may also be used as:
  • Component of homogenization buffer to homogenize adipose tissue for liquid chromatography/mass spectrometry and in HN buffer for dissolving viral stock titer after dot blot hybridization.
  • Pharmaceutical reference standard for the determination of the analyte in bulk drug and pharmaceutical formulations by high performance liquid chromatography.

Analysis Note

These secondary standards offer multi-traceability to the USP and EP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.
To see an example of a Certificate of Analysis for this material enter LRAC3253 in the slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Simple and reliable chromatographic technique for the determination of HEPES in Ga-68-labeled pharmaceuticals
Maus S, et al.
Journal of Nuclear Medicine, 56(3), 1168- 1168 (2015)
Development and evaluation of a rapid analysis for HEPES determination in 68 Ga-radiotracers
Pfaff S, et al.
EJNMMI Research, 8(1), 95-95 (2018)
FGF21 Prevents Angiotensin II-Induced Hypertension and Vascular Dysfunction by Activation of ACE2/Angiotensin-(1?7) Axis in Mice.
Pan X, et al.
Cell Metabolism (2018)
Determination of HEPES in 68 Ga-labeled peptide solutions
Sasson R, et al.
J. Radioanal. Nucl. Chem., 283(3), 753- 756 (2010)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)
View All Related Papers

Related Content

最新推出的满足海关审查要求的标准物质

默克Supelco®标准物质不断推出或更新满足海关及法规要求的标准物质。每年我们约有上百种的新CRM或标准物质上市，在此我们尽可能多的列出满足海关及法规要求的标准物质。

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service