P5288
Polyvinylpyrrolidone
for molecular biology, nucleic acid hybridization tested, mol wt 360,000
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PVP, Polyvidone, Povidone
(C6H9NO)n
Recommended Products
grade
for molecular biology
Quality Level
form
powder
mol wt
360,000
suitability
nucleic acid hybridization tested
foreign activity
Endonuclease, exonuclease, RNase, none detected
SMILES string
C=CN1CCCC1=O
InChI
1S/C6H9NO/c1-2-7-5-3-4-6(7)8/h2H,1,3-5H2
InChI key
WHNWPMSKXPGLAX-UHFFFAOYSA-N
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General description
Polyvinylpyrrolidone is a large polymer containing a carbonyl, C-N and alkyl functional groups.
Application
Polyvinylpyrrolidone has been used:
- to perform intracytoplasmic sperm injection procedure
- in the preparation of endophyte coating agent mixture, to coat creeping bentgrass seed
- as a component of prehybridization solution
Biochem/physiol Actions
Polyvinylpyrrolidone can bind to polyphenol. Thus, it is known to be used for RNA isolation from plants rich in polyphenols. It is extensively used in the synthesis of nanoparticles.
Other Notes
Polyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Blastocyst formation in in vitro fertilization versus intracytoplasmic sperm injection cycles: influence of the fertilization procedure
Fertility and Sterility, 83(5), 1397-1403 (2005)
Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
Nucleic Acids Research, 27(3), 915-916 (1999)
Current biology : CB, 30(1), 1-16 (2019-12-17)
Sleep requires sleep-active neurons that depolarize to inhibit wake circuits. Sleep-active neurons are under the control of homeostatic mechanisms that determine sleep need. However, little is known about the molecular and circuit mechanisms that translate sleep need into the depolarization
Dalton transactions (Cambridge, England : 2003), 44(41), 17883-17905 (2015-10-06)
Colloidal synthesis offers a route to nanoparticles (NPs) with controlled composition and structural features. This Perspective describes the use of polyvinylpyrrolidone (PVP) to obtain such nanostructures. PVP can serve as a surface stabilizer, growth modifier, nanoparticle dispersant, and reducing agent.
Nature, 550(7676), 393-397 (2017-10-12)
Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we
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