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P4544

Sigma-Aldrich

Phenazine ethosulfate

≥95% purity, powder

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Synonym(s):
N-Ethyldibenzopyrazine ethyl sulfate salt
Linear Formula:
C16H18N2SO4
CAS Number:
10510-77-7
Molecular Weight:
334.39
EC Number:
234-044-9
MDL number:
MFCD00050286
UNSPSC Code:
12171500
PubChem Substance ID:
329820208
NACRES:
NA.47

Quality Level

200

Assay

≥95%

form

powder

solubility

ethanol: 50 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCOS([O-])(=O)=O.CC[n+]1c2ccccc2nc3ccccc13

InChI

1S/C14H13N2.C2H6O4S/c1-2-16-13-9-5-3-7-11(13)15-12-8-4-6-10-14(12)16;1-2-6-7(3,4)5/h3-10H,2H2,1H3;2H2,1H3,(H,3,4,5)/q+1;/p-1

InChI key

VDJKJPMLWJWQIH-UHFFFAOYSA-M

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General description

Phenazine ethosulfate is a cationic dye and a chemical electron acceptor. It is used in dye-linked enzyme assays. At a high pH, the dye produces free radicals, which may be the actual electron acceptors.

Application

Phenazine ethosulfate has been used:
  • in the spectrophotometric assessment of Plasmodium falciparum lactate dehydrogenase activity
  • to study the use of the dye during culture of embryos on fetal and postnatal calf development
  • to test and validate a fluorescence approach to quantify lipid content of individual bovine oocytes and blastocysts

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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High-Throughput Screening in Drug Discovery (2006)
Palraj Kalimuthu et al.
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We report the first electrochemical study of a lanthanoid-dependent methanol dehydrogenase (Eu-MDH) from the acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV with its own physiological cytochrome cGJ electron acceptor. Eu-MDH harbours a redox active 2,7,9-tricarboxypyrroloquinoline quinone (PQQ) cofactor which is non-covalently bound
Cross-validation of techniques for measuring lipid content of
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M. Barcelo
Theriogenology (2011)
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Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

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