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Merck
CN

P4272

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt

Powder or crystals

Synonym(s):

5,5′-[3-(2-Pyridyl)-1,2,4-triazine-5,6-diyl]difuran-2-sulfonic acid disodium salt

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About This Item

Empirical Formula (Hill Notation):
C16H8N4Na2O8S2
CAS Number:
79551-14-7
Molecular Weight:
494.37
NACRES:
NA.47
PubChem Substance ID:
24898520
UNSPSC Code:
12171500
MDL number:
MFCD00040642
Beilstein/REAXYS Number:
5710932

Key Documents

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Product Name

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, Suitable for determination of Fe

InChI

1S/C16H10N4O8S2.2Na/c21-29(22,23)12-6-4-10(27-12)14-15(11-5-7-13(28-11)30(24,25)26)19-20-16(18-14)9-3-1-2-8-17-9;;/h1-8H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc(o1)-c2nnc(nc2-c3ccc(o3)S([O-])(=O)=O)-c4ccccn4

InChI key

IVLSEFOVPQFJBB-UHFFFAOYSA-L

form

powder or crystals

composition

Water: ≤ 13.0%

Carbon (Theory), anhydrous: 38.87%

Nitrogen (Theory), anhydrous: 11.33%

technique(s)

titration: suitable

color

white to dark yellow

solubility

water: 50 mg/mL, clear to slightly hazy

cation traces

C: 38.87% ((Theory), anhydrous)
N: 11.33% ((Theory), anhydrous)

suitability

suitable for determination of iron

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt has been used as a component in a sensitive colorimetric assay (ferene-s assay) for the detection of iron. It has also been used as a ligand to study the complexation towards copper(II).

General description

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, also known as Ferene, is a chelating agent commonly used to measure the concentration of iron ions in aqueous solutions. The reagent is sensitive to ferrous iron ions (Fe2+) and forms a purple-colored complex with these ions in the presence of a reducing agent. The intensity of the purple color formed is proportional to the concentration of iron ions present in a solution and is quantified spectrophotometrically.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9759
BCCN9275
BCCN3166
BCCM6745
BCCL3263

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Synthesis, Characterization, and Antimicrobial Activity of Novel Sulfonated Copper?Triazine Complexes.
Katugampala S, et al.
Bioinorganic Chemistry and Applications, 2530851-2530851 (2018)
A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens.
Abbasi U, et al.
Scientific Reports, 6008, 11(1)-11(1) (2021)
C Charlier et al.
Annales de biologie clinique, 50(3), 191-202 (1992-01-01)
The previously selected method (1977) for the measurement of iron in human serum has been modified by the working group on Iron and Iron-Transport Proteins (Sociéte Française de Biologie Clinique). Ferene S is used as the chromogen, it is more
M C Chung
Analytical biochemistry, 148(2), 498-502 (1985-08-01)
A new method for specifically staining the iron atoms present in transferrin and lactoferrin after polyacrylamide gel electrophoresis and isoelectric focusing is described. The stain, 3-(2-pyridyl)-5,6-bis(2-(5-furylsulfonic acid))-1,2,4-triazine, disodium salt, or Ferene S, will detect transferrin in 5 microliters of human
Darius J R Lane et al.
Analytical biochemistry, 373(2), 287-295 (2007-10-24)
Ferricyanide reduction frequently is analyzed to determine the activity of membraneous reductases. An improved, highly sensitive, and rapid method for quantitative endpoint determination of ferrocyanide is presented. Ferrocyanide is oxidized by Fe(3+) in the presence of Ferene-S under acid conditions
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