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P1754

Sigma-Aldrich

TWEEN® 80

viscous liquid

Synonym(s):

TWEEN® 80, POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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About This Item

CAS Number:
9005-65-6
MDL number:
MFCD00082107
UNSPSC Code:
12161900
NACRES:
NA.21

biological source

Porcine (tallow)
bovine (tallow)

Quality Level

200

description

non-ionic

form

viscous liquid

mol wt

micellar avg mol wt 79,000
average mol wt 1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

CMC

0.012 mM (20-25°C)

viscosity

400-620 mPa.s

transition temp

cloud point 65 °C

solubility

water: soluble, clear to slightly hazy, colorless to yellow

density

1.064 g/cm3

HLB

15

application(s)

cell analysis

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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General description

Tween® 80 is a non-ionic detergent and surfactant, classified as an ester of sorbitan and oleic acid. It is a versatile compound commonly employed in various biological applications, including cell lysis, nuclei isolation, and cell fractionation. Additionally, Tween® 80 finds use in stabilizing proteins and facilitating protein membrane studies. Its effectiveness stems from its membership in the polysorbate group, characterized by the combination of sorbitol and sorbitane oleic esters with polyethylene glycol chains. These structural features contribute to Tween® 80′s emulsifying capabilities and a broad range of applications.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.

Biochem/physiol Actions

TWEEN® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells.

Features and Benefits

  • Excellent wetting and dispersing agent
  • Highly versatile surfactant for your Cell Biology and Biochemical research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

WGK

WGK 1

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the Critical Micelle Concentration (CMC) of Product P1754, TWEEN® 80?

    The CMC value is 13-15 mg/Liter. 

  4. Is Product P1754, TWEEN® 80, sterile?

    The product is not sterile. Autoclaving a solution of TWEEN 80 is generally not advised. Sterile filtration is more easily done if the liquid is warmed to about 40°C and alternate portions of hot distilled water and TWEEN 80 are poured through the 0.22 μm filter. The TWEEN 80 will blend and remain in solution.

  5. Is Product P1754, TWEEN® 80, cell culture tested?

    No, P1754 is not cell culture tested, but product P4780 is cell culture tested TWEEN 80.

  6. Is Product P1754, TWEEN® 80, tested for RNase and DNase activity?

    No. Product P1754 is not tested for contaminating RNase and DNase activity.  Product P5188 is a Molecular Biology grade product that has been tested for DNase and RNase.

  7. Is it normal for the color of Product P1754, TWEEN® 80, to change from lot to lot?

    Yes. According to Sigma-Aldrich's specifications, Product No. P1754 can appear bright yellow to yellow with a green cast.  Please see our Specification Sheet for more information.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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