P0532
Phthaldialdehyde Reagent
Solution Complete
Synonym(s):
OPA
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About This Item
CAS Number:
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352201
MDL number:
Product Name
Phthaldialdehyde Reagent, Solution Complete
InChI
1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
SMILES string
[H]C(=O)c1ccccc1C([H])=O
InChI key
ZWLUXSQADUDCSB-UHFFFAOYSA-N
storage temp.
2-8°C
Quality Level
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Application
Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.
Other Notes
Contains 1 mg o-phthaldialdehyde (P0657) per mL solution with 2-mercaptoethanol as the sulfhydryl moiety.
signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - Skin Sens. 1
wgk
WGK 3
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Silke B Lohan et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-25)
The daily consumption of tobacco products leads to a boost in free radical production in tissues, promoting the risk for malignancies, metabolic alterations and chronic-inflammatory diseases. This study aimed to broaden the knowledge of the status of the antioxidative (AO)
Jin-Aa Oh et al.
Journal of chromatography. A, 1247, 99-103 (2012-06-12)
A simple high performance liquid chromatographic (HPLC) method and a highly sensitive gas chromatography mass spectrometric (GC-MS) method have been established for the determination of ortho-phthalaldehyde (OPA) in water. These methods are based on the derivatization of OPA with hydrazine
Tomáš Roušar et al.
Analytical biochemistry, 423(2), 236-240 (2012-03-06)
Glutathione is an important tripeptide involved in a variety of cellular processes. Thus, precise knowledge of its levels is essential. Glutathione exists in two free forms-reduced and oxidized-and a number of methods exist to measure its levels. The aim of
Katsuhiro Suzuki et al.
Biomedical chromatography : BMC, 27(4), 535-538 (2012-09-29)
Monomethylarginine, asymmetric dimethylarginine and symmetric dimethylarginine were separated on a Wakopak Combi ODS with an acetonitrile-100 mm potassium phosphate buffer (pH 7.0; 1:1, v/v). Dimethylarginines were derived from o-phthalaldehyde for the fluorescence detector and from 6-ferrocenyl-1-hexanethiol for the electrochemical detector. The
Vishnu Menon et al.
Journal of fluorescence, 23(2), 311-321 (2012-12-06)
This is the first report of inactivation of xyloglucanase from Thermomonospora sp by pepstatin A, a specific inhibitor towards aspartic proteases. The steady state kinetics revealed a reversible, competitive, two-step inhibition mechanism with IC 50 and K i values of
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