P0532
Phthaldialdehyde Reagent
Solution Complete
Synonym(s):
OPA
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About This Item
CAS Number:
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352201
MDL number:
InChI
1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
SMILES string
[H]C(=O)c1ccccc1C([H])=O
InChI key
ZWLUXSQADUDCSB-UHFFFAOYSA-N
storage temp.
2-8°C
Quality Level
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Application
Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.
Other Notes
Contains 1 mg o-phthaldialdehyde (P0657) per mL solution with 2-mercaptoethanol as the sulfhydryl moiety.
signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - Skin Sens. 1
wgk
WGK 3
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Silke B Lohan et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-25)
The daily consumption of tobacco products leads to a boost in free radical production in tissues, promoting the risk for malignancies, metabolic alterations and chronic-inflammatory diseases. This study aimed to broaden the knowledge of the status of the antioxidative (AO)
A Pappa-Louisi et al.
Talanta, 85(4), 2241-2245 (2011-08-30)
In an effort to enhance complex mixture separations by using small amounts of a homologous series of alkanols as additives in the mobile phases, it was proposed an optimization algorithm based on a sixth-parameter retention model. This model considers simultaneously
Jong-Suk Song et al.
Investigative ophthalmology & visual science, 53(10), 6164-6170 (2012-08-18)
We evaluated whether viscoelastics have protective effects on the corneal endothelial cell damage in a toxic anterior segment syndrome (TASS) animal model depending on the types of viscoelastics. A TASS animal model was established with an injection of 0.1 mL
Jianhua Liu et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 91, 307-313 (2012-03-06)
Relying on the reaction of o-phthalaldehyde (OPA) with glutathione (GSH) to form a highly fluorescence derivative GSH-OPA has been widely used to measure reduced glutathione. In order to better understand spectra property of the GSH-OPA and the effect of zinc
Quansong Li et al.
Physical chemistry chemical physics : PCCP, 14(18), 6561-6568 (2012-03-30)
The potential energy surface for the intramolecular excited state hydrogen transfer (IESHT) in ortho-phthalaldehyde (OPA), which generates an enol ketene, has been studied with ab initio calculations (MS-CASPT2//CASSCF). The goal of our study is to establish the mechanistic factors that
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