Column performance test mixture of liquid chromatography

This Standard Reference Material (SRM) is a mixture of five organic compounds in methanol and is intended for use in characterizing general aspects of liquid chromatographic (LC) column performance, including efficiency, void volume, methylene selectivity, retentiveness, and activity toward chelators and organic bases. Other possible uses include column classification to aid column selection during method development, as a control material for monitoring LC column performance over time, and in quality control for column manufacturing.

SRM 870 consists of a mixture of the following five organic compounds in methanol: uracil, toluene, ethyl benzene, quinizarin, and amitriptyline (added as amitriptyline hydrochloride; see Figure 1 for structures). A unit of SRM 870 consists of five ampoules each containing approximately 1.1 mL of the mixture. For more information, please refer to the SDS and COA.

SRM 870_cert

SRM 870_SDS

Certified for the analytes listed below.
Uracil (C₄H₄N₂O₂), Toluene (C₆H₅CH₃), Ethyl Benzene (C₈H₁₀), Quinizarin (C₁₄H₈O₄), Amitriptyline HCl (C₂₀H₂₄ClN), Methanol (CH₃OH)

Reference/Informational value is provided for the following analytes.
Uracil (C₄H₄N₂O₂), Toluene (C₆H₅CH₃), Ethyl Benzene (C₈H₁₀), Quinizarin (C₁₄H₈O₄), Amitriptyline HCl (C₂₀H₂₄ClN), Methanol (CH₃OH)

See certificate for values and more details at nist.gov/SRM.

NIST is a registered trademark of National Institute of Standards and Technology

SRM is a registered trademark of National Institute of Standards and Technology