NIST2141
Urea
NIST® SRM® 2141
Synonym(s):
Carbamide, Carbonyldiamide
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About This Item
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
MDL number:
UNSPSC Code:
41116107
PubChem Substance ID:
NACRES:
NA.24
grade
certified reference material
Quality Level
form
crystals
packaging
pkg of 2 g
manufacturer/tradename
NIST®
mp
132-135 °C (lit.)
density
1.335 g/mL at 25 °C (lit.)
application(s)
clinical testing
SMILES string
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
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General description
Application
Urea Standard reference material (SRM) is certified for calibrating and standardizing microchemical procedures aimed at determining nitrogen in organic matter. Additionally, it serves as a tool for standardizing techniques for nitrogen determination, when carbon and hydrogen are simultaneously analyzed.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Features and Benefits
Available with a certificate documentingnitrogen value determined through a modification of the AOAC micro-Kjeldahlmethod.
Other Notes
Example analytes are listed below as a reference. Please download a current certificate at nist.gov/SRM for current analytes and certified values.
Nitrogen (N)
See certificate for values and more details at nist.gov/SRM.
Nitrogen (N)
See certificate for values and more details at nist.gov/SRM.
Legal Information
NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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