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N7005

Sigma-Aldrich

Neutral Red

Dye content ≥90 %, Powder

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Synonym(s):
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
Colour Index Number:
50040
Beilstein:
3918740
EC Number:
209-035-8
MDL number:
MFCD00012651
UNSPSC Code:
12171500
PubChem Substance ID:
24897804
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, ≥90%

technique(s)

titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

water: 10 mg/mL, dark red

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Application

Neutral red has multiple staining applications that include its use as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture). Neutral red staining is also a viability assessment method used for the detection of viable yeast cells in dandruff samples and of mussel larvae. Neutral red is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells. It is also used for supravital staining of living blood cells.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Quality

Purified

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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