Skip to Content
Merck
CN
All Photos(3)

Documents

M8884

Sigma-Aldrich

Methyl Green

~85% purity, powder

Synonym(s):

Ethyl Green

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
Colour Index Number:
42590
Beilstein:
3914106
EC Number:
230-415-4
MDL number:
MFCD00151094
UNSPSC Code:
12171500
PubChem Substance ID:
24897293
NACRES:
NA.47

product name

Methyl Green, zinc chloride salt, ~85%

Quality Level

200

Assay

~85%

form

powder

composition

Dye Content, ≥83%

color

red to very dark red

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 1 mg/mL

εmax

>11100.00 at 417-425 nm in water at 0.005 g/L
>16000 at 311-319 nm in water at 0.005 g/L
>60000 at 626-634 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Application

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Quality

Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts. Halogen contents listed on label.

Analysis Note

Dye content based on molecular weight of monobromo-monochloro ZnCl2 salt (653.2).

Signal Word

Danger

Hazard Statements

H314,H335,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A new semi-selective medium for Xanthomonas campestris pv. vitians, the causal agent of bacterial leaf spot of lettuce.
Toussaint V, et. al.
Plant Disease, 85, 131-131 (2001)
Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals.
Li WC
The Journal of Cell Biology, 130, 169-169 (1995)
International Review of Cytology (1955)
Expression of the axonal membrane glycoprotein M6a is regulated by chronic stress.
Cooper B
PLoS ONE, 4, e3659-e3659 (2009)
Alena Rudkouskaya et al.
Theranostics, 10(22), 10309-10325 (2020-09-16)
Rationale: Following an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imaging offers
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service