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L1886

Sigma-Aldrich

Light Green SF Yellowish

Dye content, ≥65%, certified by the Biological Stain Commission, powder

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Synonym(s):
Acid Green 5
Linear Formula:
C37H34N2O9S3Na2
CAS Number:
5141-20-8
Molecular Weight:
792.85
Colour Index Number:
42095
Beilstein:
5717828
EC Number:
225-906-5
MDL number:
MFCD00012121
UNSPSC Code:
12171500
PubChem Substance ID:
24896295
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥65%

color

red to dark purple

mp

288 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O

InChI

1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

InChI key

DGOBMKYRQHEFGQ-UHFFFAOYSA-L

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General description

Light Green SF Yellowish or Acid Green 5 is a triarylmethane dye that exists as disodium salt. It has the absorption value of 629 nm.

Application

Light Green SF Yellowish functions as an efficient histological stain for collagen. It is also used as colorant for foodstuff and beverages.
Light Green SF Yellowish has been used to determine surface structures of trypsin treated tissue sections and to stain buccal cells for slide scoring.

Biochem/physiol Actions

Light Green SF Yellowish functions as an efficient histological stain for collagen. It is also used as colorant for foodstuff and beverages.

Suitability

Certified for use as a cytological counterstain and as a substitute for aniline blue in Gomori′s one-step trichrome procedure.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Buccal micronucleus cytome biomarkers may be associated with Alzheimer's disease.
Thomas P
Mutagenesis, 22, 371-371 (2007)
Atomic force microscope investigation of the boundary-lubricant layer in articular cartilage.
Chan SM
Osteoarthritis and Cartilage, 18, 956-956 (2010)
Atomic force microscope investigation of the boundary-lubricant layer in articular cartilage
Chan SMT, et al.
Osteoarthritis and Cartilage, 18(7), 956-963 (2010)
The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls
Thomas P, et al
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 638, 37-47 (2008)
Decoloration treatment of a hazardous triarylmethane dye, Light Green SF (Yellowish) by waste material adsorbents
Mittal A, et al.
Journal of Colloid and Interface Science, 342(2), 518-527 (2010)
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