I8377
Iodonitrotetrazolium chloride
Used in colorimetric assays.
Synonym(s):
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT
About This Item
Recommended Products
Quality Level
Assay
≥98% (TLC)
form
powder
mp
240 °C (dec.) (lit.)
solubility
H2O: 4 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4
InChI
1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChI key
JORABGDXCIBAFL-UHFFFAOYSA-M
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Application
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Sol. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
4.1B - Flammable solid hazardous materials
WGK
WGK 3
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Product I8377, Iodonitrotetrazolium chloride, is stated to have solubility in water, ethanol and ethylene glycol monomethylether. What other solutions can be used?
Conn's Biological Stains, 10th Edition (page 163) states P-Iodonitrotetrazolium Violet is also soluble in dimethylsulfoxide and dimethylsulfoxide-water mixtures.
Can Product I8377, Iodonitrotetrazolium chloride, be used for the staining of cells?
Yes, although due to the large crystal size of the formazan and lack of substantivity, histological application of I8377 has been limited. In microbiology, the product was used by Lussenhop and Fogel (1993) for in situ microscopic observation of soil biota.. Gribbon and Barer (1995) used for the demonstration of viable but nonculturable bacteria. Heidelberg (1997) used as a bacterial viability stain.
Is Product I8377, Iodonitrotetrazolium chloride, lipophilic?
Yes, I8377 is a lipophilic cation of moderate size. The resulting formazan is more strongly liophilic and fat soluble.
Is there a procedure for reducing Product I8377, Iodonitrotetrazolium chloride, by superoxide radicals?
Yes. See Biochem Biophys Res Commun. 1988 Feb 15;150(3):1294-301.
Is there a reference for using Product I8377, Iodonitrotetrazolium chloride, to treat brain membrane preparations?
Yes. See Neuroscience Letters Volume 35, Issue 2, 21 February 1983, Pages 215-220
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