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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonym(s):

Ready-to-use density gradient media

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About This Item

CAS Number:
26873-85-8
MDL number:
MFCD00081599
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

0.2 μm filtered

Quality Level

200

type

Type 400

form

liquid

concentration

20% in H2O

color

clear colorless

solubility

water: soluble

density

1.055-1.075 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product F5415, Ficoll® solution 20% suitable for the separation of mononuclear cells without further dilution?

    No.  The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL.  Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL.  Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical.  Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.

  6. Is Product F5415, Ficoll® solution 20%, sterile?

    No claims are made for sterility for F5415.  If a sterile product is required for cell culture use,  product F8636 has been gamma irradiated and is considered a sterile product.  Product F8636 is a powder and does not come as a sterile solution.

  7. What is the difference between Product F5415, Ficoll® solution 20%, and the polysucrose type 400 used in the Histopaque 1077-1?

    Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark.  Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.

  8. Can more Ficoll be added to Product F5415, Ficoll® solution 20%, to increase the density to 1.077 g/mL?

    This is not recommended.  Ficoll has a molecular weight in the range of 400,000.  Even 20% solutions of such high molecular weight products have extremely low osmolality.  Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic.  Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.

  9. Is there a Product Information Sheet for Product F5415, Ficoll® solution 20%?

    No.  However there is a Product Information Sheet for product F4375.  This product information sheet gives general information regarding Ficoll and possible uses.  If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.

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