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F0763

Sigma-Aldrich

Fura 2 pentapotassium salt

≥80% purity, powder

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Empirical Formula (Hill Notation):
C29H22K5N3O14
CAS Number:
113694-64-7
Molecular Weight:
831.99
MDL number:
MFCD00083328
UNSPSC Code:
12171500
PubChem Substance ID:
24894731
NACRES:
NA.47

Quality Level

100

Assay

≥80%

form

powder

color

yellow

solubility

1 M NaOH: 10 mg/mL

εmax

24-37 at 363 nm (pH 7.2)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc3cc(oc3cc2N(CC([O-])=O)CC([O-])=O)-c4ncc(o4)C([O-])=O)c1

InChI

1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40;;;;;/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42);;;;;/q;5*+1/p-5

InChI key

HQKWBBCTCLUGSX-UHFFFAOYSA-I

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General description

Fura 2 pentapotassium salt (fura-2) is a fluorescent ratiometric Ca2+ dye. Fura 2 pentapotassium salt (fura-2) helps to estimate the level of cytosolic Ca(2+) by determining the emission found at 510 nm after alternating excitation at 340 nm and 380 nm (F340/F380 ratio). Hence, it is considered as the standard for quantitative intracellular calcium measurements.

Application

Cell impermeable fluorescent probe for Ca2+.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Resveratrol interferes with fura-2 intracellular calcium measurements
Kopp, et al.
Journal of Fluorescence, 24(2), 279-284 (2014)
Ram Chandra Paudel et al.
Biochemical and biophysical research communications, 450(4), 1626-1630 (2014-07-19)
Fura-2 is a commonly used fluorescent Ca(2+) dye that allows an accurate determination of cytosolic Ca(2+) levels by measuring the emission obtained at 510 nm after alternating excitation at 340 nm and 380 nm (F340/F380 ratio). Previous studies, based on

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