Skip to Content
Merck
CN
All Photos(3)

Documents

E4884

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

ACS reagent, 99.0-101.0%

Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2
Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
6381-92-6
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
205-358-3
UNSPSC Code:
12352107
PubChem Substance ID:
24894586
NACRES:
NA.77

grade

ACS reagent

Quality Level

200

Agency

suitable for SM 4500 - NH3

Assay

99.0-101.0%

form

solid

reaction suitability

reagent type: chelator

impurities

≤0.005% Insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

pH

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

solubility

H2O: 100 g/L at 20 °C

cation traces

Fe: ≤0.01%
heavy metals: ≤0.005% (by ICP-OES)

application(s)

microbiology

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.
Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:
  • of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
  • of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
  • of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Other Notes

For additional information on our range of Biochemicals, please complete this form.

comparable product

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H332,H373

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts
Sin J, et al.
Autophagy, 12(2), 369-380 (2016)
Conformational landscape of the p28-bound human proteasome regulatory particle
Lu Y, et al.
Molecular Cell, 67(2), 322-333 (2017)
Human Cardiomyocyte Progenitor Cells in Co-culture with Rat Cardiomyocytes Form a Pro-arrhythmic Substrate: Evidence for Two Different Arrhythmogenic Mechanisms
Smit NW, et al.
Frontiers in Physiology, 8, 797-797 (2017)
Does calcium disodium EDTA slow CKD progression?
Weaver VM, et al.
American Journal of Kidney Diseases, 60(4), 503-506 (2012)
Lysozyme/EDTA disodium salt and modified-atmosphere packaging to prolong the shelf life of burrata cheese
Conte A, et al.
Journal of Dairy Science, 94(11), 5289-5297 (2011)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service