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E4009

Sigma-Aldrich

Eosin Y

Dye content ~99%, Powder

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43

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About This Item

Empirical Formula (Hill Notation):
C20H8Br4O5
CAS Number:
15086-94-9
Molecular Weight:
647.89
Colour Index Number:
45380:2
EC Number:
239-138-3
MDL number:
MFCD00036189
UNSPSC Code:
12171500
PubChem Substance ID:
24894536
NACRES:
NA.47

product name

Eosin Y, Dye content ~99 %

form

powder

Quality Level

200

composition

Dye content, ~99%

color

light red to red/pink

solubility

ethanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

DBZJJPROPLPMSN-UHFFFAOYSA-N

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Application

Eosin Y has been used as a stain:
  • to determine the location of the brain sections
  • for staining of fixed liver tissue
  • for histological examination of brain sections from the dorsal hippocampus

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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An immunohistochemical analysis of SERT in the blood-brain barrier of the male rat brain.
Young LW, et al.
Histochemistry and Cell Biology, 144, 321-321 (2015)
Antonello Mai et al.
Journal of medicinal chemistry, 51(7), 2279-2290 (2008-03-20)
A number of new compounds bearing two ortho-bromo- and ortho, ortho-dibromophenol moieties linked through a saturated/unsaturated, linear/(poly)cyclic spacer (compounds 1- 9) were prepared as simplified analogues of AMI-5 (eosin), a recently reported inhibitor of both protein arginine and histone lysine
Florian Ménard et al.
Bioorganic & medicinal chemistry, 17(22), 7647-7657 (2009-10-13)
This paper reports the synthesis and characterization of a new class of tetrabenzoporphyrins bearing glucosyl or polyamine units on meso positions to improve the targeting of cancer cells. Photocytotoxic activity of these photosensitizers was tested on cell lines HaCaT and
The effects of simvastatin on hippocampal caspase-3 and Bcl-2 expression following kainate-induced seizures in rats.
Sun J, et al.
International Journal of Molecular Medicine, 30, 739-739 (2012)
Inactivation of NF-kappaB p50 leads to insulin sensitization in liver through post-translational inhibition of p70S6K.
Gao Z, et al.
The Journal of Biological Chemistry, 284, 18368-18368 (2009)
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