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Merck
CN

E1769

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

BioXtra

Synonym(s):

N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC, EDC hydrochloride, WSC hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C8H17N3 · HCl
CAS Number:
25952-53-8
Molecular Weight:
191.70
NACRES:
NA.25
PubChem Substance ID:
24894433
UNSPSC Code:
12161700
EC Number:
247-361-2
MDL number:
MFCD00012503
Beilstein/REAXYS Number:
5764110

Key Documents

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Product Name

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, BioXtra

InChI key

FPQQSJJWHUJYPU-UHFFFAOYSA-N

InChI

1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H

SMILES string

Cl.CCN=C=NCCCN(C)C

product line

BioXtra

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

mp

110-115 °C (lit.)

solubility

H2O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow

anion traces

sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

storage temp.

−20°C

Quality Level

200

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Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
  • for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
  • for cross-linking of nanofiber matrices with mouse lung extract
  • for activation of substrate XNA oligonucleotides

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Stomach,large intestine,lymph node

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL2739
BCCL9785
BCCM1868
0000281958
SLCG9487

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Organ-derived coatings on electrospun nanofibers as ex vivo microenvironments
Fischer SN, et al.
Biomaterials, 32(2), 538-546 (2011)
Directed evolution of artificial enzymes (XNAzymes) from diverse repertoires of synthetic genetic polymers
Taylor AI and Holliger P
Nature Protocols, 10(10), 1625-1625 (2015)
Henry H Lee et al.
Nature communications, 10(1), 2383-2383 (2019-06-05)
DNA is an emerging medium for digital data and its adoption can be accelerated by synthesis processes specialized for storage applications. Here, we describe a de novo enzymatic synthesis strategy designed for data storage which harnesses the template-independent polymerase terminal
Amrita D Chaudhuri et al.
Frontiers in cellular neuroscience, 7, 160-160 (2013-09-26)
Identification of the cell type of origin for normal or aberrant gene expression is critical for many studies, and poses a significant problem for some regulatory RNAs such as microRNAs. MicroRNAs are small non-coding RNAs that regulate cellular function by
Francesca Paradiso et al.
Frontiers in bioengineering and biotechnology, 7, 343-343 (2020-01-11)
A fundamental structural component of extracellular matrix in all connective and interstitial tissue, collagen is the most abundant protein in the human body. To date, mammalian collagens sources represent the golden standard for multiple biomedical applications, while marine-derived collagens have
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