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DLW356231

Sigma-Aldrich

Corning® Matrigel® Matrigel Matrix GFR PhenolRF Mouse 10 mL

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About This Item

UNSPSC Code:
12352207

shipped in

dry ice

storage temp.

−20°C

General description

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

Quality
  • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing.
  • Extensive PCR testing is performed to screen for a number of pathogens.
  • Tested and found negative for bacteria, fungi, and mycoplasma.
  • Protein concentrations determined by Lowry method.
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay.
  • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C.
  • Biological activity is determined for each lot using a neurite outgrowth assay.

Other Notes

Source:
Engelbreth-Holm-Swarm Mouse Tumor.

Formulation:
High concentration laminin/entactin complex frozen in Dulbecco′s PBS.

Legal Information

Corning is a registered trademark of Corning, Inc.
Matrigel is a registered trademark of Corning, Inc.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Muhammedin Deliorman et al.
Bio-protocol, 10(18), e3764-e3764 (2021-03-05)
This protocol describes a simple method to cryopreserve mammalian cells within filter papers as an alternative to conventional slow-freezing approach. The method involves treating paper fibers with fibronectin, using low concentrations of the cryoprotectant dimethyl sulfoxide (DMSO), and slow freezing

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