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Merck
CN

D9805

Fast Blue B Salt

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

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About This Item

Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
14263-94-6
Molecular Weight:
475.47
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894113
EC Number:
238-153-2
MDL number:
MFCD00075203
Colour Index Number:
37235
Beilstein/REAXYS Number:
1499067

Key Documents

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Product Name

Fast Blue B Salt, Dye content ~95 %

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

description

Technical grade

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

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Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

pictograms

FlameHealth hazard
GHS02,GHS08

signalword

Danger

hcodes

H242,H350

Hazard Classifications

Carc. 1B - Self-react. C

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
SHBT3017
MKCK4645
MKCG1594
MKCC1010
MKBZ2692V

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Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata).
Nolasco H, et al.
Fish Physiology and Biochemistry, 37, 43-43 (2011)
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
Sartory DP, et al.
Letters in Applied Microbiology, 42, 418-418 (2006)
A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
Sim?es-Pires CA, et al.
Phytochemical Analysis, 20, 511-511 (2009)
Dong Ho Kang et al.
Polymers, 12(11) (2020-11-18)
In this study, composite anion exchange membranes (AEMs) were synthesized by cross-linking poly(phenylene oxide) (PPO) with cellulose functionalized by 1,4-diazabicyclo[2.2.2]-octane (DABCO) or di-guanidine (DG). The structural and morphological characteristics of the synthesized AEMs were characterized by FTIR, 1H-NMR, SEM, TEM
Overcoming the bell-shaped dose-response of cannabidiol by using Cannabis extract enriched in cannabidiol.
Gallily R, et al.
Pharmacology & Pharmacy, 6, 75-75 (2015)
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