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D9805

Sigma-Aldrich

Fast Blue B Salt

Dye content ~95 %, Powder

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Synonym(s):
o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B
Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
14263-94-6
Molecular Weight:
475.47
Colour Index Number:
37235
Beilstein:
1499067
EC Number:
238-153-2
MDL number:
MFCD00075203
UNSPSC Code:
12171500
PubChem Substance ID:
24894113
NACRES:
NA.47

description

Technical grade

Quality Level

200

form

powder

composition

Dye content, ~95%

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

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Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

Pictograms

FlameHealth hazard
GHS02,GHS08

Signal Word

Danger

Hazard Statements

H242,H350

Hazard Classifications

Carc. 1B - Self-react. C

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dong Ho Kang et al.
Polymers, 12(11) (2020-11-18)
In this study, composite anion exchange membranes (AEMs) were synthesized by cross-linking poly(phenylene oxide) (PPO) with cellulose functionalized by 1,4-diazabicyclo[2.2.2]-octane (DABCO) or di-guanidine (DG). The structural and morphological characteristics of the synthesized AEMs were characterized by FTIR, 1H-NMR, SEM, TEM
A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
Sim?es-Pires CA, et al.
Phytochemical Analysis, 20, 511-511 (2009)
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
Sartory DP, et al.
Letters in Applied Microbiology, 42, 418-418 (2006)
Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata).
Nolasco H, et al.
Fish Physiology and Biochemistry, 37, 43-43 (2011)
D L Oblack et al.
Sabouraudia, 21(3), 243-245 (1983-09-01)
Trichosporon isolates form pink to violet pigments following reaction with diazonium blue B which reliably differentiates them from Geotrichum species. The inoculum could be grown in static culture without diminishing the colour intensity. This modification facilitates use of the diazonium
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