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C9386

Millipore

Hy-Case® SF

Casein acid hydrolysate, from bovine milk

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Synonym(s):
Casein hydrolyzates, Peptone from casein, casein acid hydrolysate
CAS Number:
65072-00-6
EC Number:
265-363-1
UNSPSC Code:
41106212
NACRES:
NA.85

biological source

bovine milk

Quality Level

200

form

powder

nitrogen analysis

8-12% amino, ≥12.5% total

loss

≤11% loss on drying

pH

5.4-7.4

application(s)

food and beverages
microbiology

storage temp.

10-30°C

suitability

Streptococcus spp.
Streptomyces spp.

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General description

Hy-Case® SF is a high-quality acid hydrolysis of casein. Casein is a rich source of amino nitrogen so on treatment with a strong acid, it gets hydrolysed, producing free amino acids and several short peptide fragments. Special processing keeps inorganic ions to a minimum, so Hy-Case® SF is virtually a high concentration of free amino acids with a minimum of salt. It is a refined grade hydrolysate with high clarity in solution and is food grade and is recommended where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.

Application

Casein hydrolysate Hy-Case® SF was used in DMCY medium, during an experimental study conducted to investigate the role of ssgA gene product in promoting the sporulation and cell division in Streptomyces griseus. It may also be used in the YH medium in studies related to Halobacterium denitrificans sp. which is characterised as being carbohydrate-utilizing, denitrifying, extremely halophilic bacteria.
Recommended for use as a microbiological nutrient in laboratory media, fermentations, and tissue culture applications requiring a mixture of highly soluble free amino acids.

Linkage

Similar to Hy-Case Amino, but contains a maximum of 2.0% sodium chloride

Reconstitution

Recommended concentration: 25 g/L

Legal Information

Hy-Case is a registered trademark of Kerry Group

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How are the Amicase® (product A2427) and Hy-Case® SF (product C9386) different?

    The Amicase® (product A2427) and Hy-Case® SF (product C9386) are related items, but they are not exactly the same.  The Amicase® is prepared to keep the inorganic ions to a minimum so it is high in total nitrogen.  This is also the case for the Hy-Case® SF; however, this item is prepared to be essentially salt free (contains a maximum of 2.0% sodium chloride).  The Hy-Case® SF is recommended for use as a microbiological nutrient in laboratory media and fermentations, where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.

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S Kawamoto et al.
Microbiology (Reading, England), 143 ( Pt 4), 1077-1086 (1997-04-01)
The ssgA gene of Streptomyces griseus B2682, when present in high copy number, results in both suppression of sporulation and fragmented growth of mycelia. Western analysis with polyclonal antibodies against the gene product (SsgA) revealed a close correlation between SsgA
G A Tomlinson et al.
International journal of systematic bacteriology, 36(1), 66-70 (1986-01-01)
Halobacterium denitrificans was one of several carbohydrate-utilizing, denitrifying, extremely halophilic bacteria isolated by anaerobic enrichment in the presence of nitrate. Anaerobic growth took place only when nitrate (or nitrite) was present and was accompanied by the production of dinitrogen.

Articles

Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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