Skip to Content
Merck
CN
All Photos(4)

Documents

C1144

Sigma-Aldrich

Chlorazol Black

Powder

Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38
Empirical Formula (Hill Notation):
C34H25N9Na2O7S2
CAS Number:
1937-37-7
Molecular Weight:
781.73
Colour Index Number:
30235
EC Number:
217-710-3
MDL number:
MFCD00066363
UNSPSC Code:
12171500
PubChem Substance ID:
24892361
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, ≥45% (based on Nitrogen)

color

dark brown to black

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1

InChI

1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;

InChI key

XRPLBRIHZGVJIC-UVHCWRHYSA-L

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin. It also helps in distinguishing fungal bodies from artefacts due to chitin staining. It is also used as an auxiliary stain for chromosomes.

Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H319,H350,H361d

Hazard Classifications

Carc. 1B - Eye Irrit. 2 - Repr. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

R P Bos et al.
Toxicology, 40(2), 207-213 (1986-08-01)
The role of the rat intestinal flora in the azo reduction of some benzidine-based dyes was studied in vitro and in vivo. The formation of benzidine was measured after anaerobic incubation of direct black 38, direct blue 6 and direct
Mercedes Conradi et al.
ZooKeys, (791), 71-95 (2018-11-06)
Spaniomolgus is a symbiotic genus of copepods of the poecilostomatoid family Rhynchomolgidae and is known to be associated with shallow-water reef-building hermatypic corals. Three species of this genus were previously found only in washings of Acropora and Stylophora in northern
Mutagenic activation of the benzidine-based dye direct black 38 by human intestinal microflora.
C E Cerniglia et al.
Mutation research, 175(1), 11-16 (1986-09-01)
Jianyong An et al.
The New phytologist, 224(1), 396-408 (2019-05-31)
Plants form a mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi, which facilitates the acquisition of scarce minerals from the soil. In return, the host plants provide sugars and lipids to its fungal partner. However, the mechanism by which the AM
Baran R, et al.
Onychomycosis: The Current Approach to Diagnosis and Therapy (1999)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service