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B8806

Sigma-Aldrich

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

1 mg/mL in DMSO

Synonym(s):

BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid

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About This Item

EC Number:
200-664-3
MDL number:
MFCD00036969
UNSPSC Code:
12171500
NACRES:
NA.47

description

Mixture of six isomeric compounds

Quality Level

200

form

liquid

concentration

1 mg/mL in DMSO

color

colorless to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O

InChI

1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3

InChI key

NTECHUXHORNEGZ-UHFFFAOYSA-N

Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.

Biochem/physiol Actions

An intracellular fluorescent pH indicator.
Hydrolyzed to BCECF by cytosolic esterases.

Physical form

Solution in 0.1% BCECF AM Ester and 99.9% DMSO

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

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Certificates of Analysis (COA)

Lot/Batch Number
SLCR2404
SLCN9375
SLCN9373
SLCD5545
SLBZ8252

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