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Merck
CN

B1256

Bromocresol Green sodium salt

≥98% purity, crystalline

Synonym(s):

3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C21H13Br4NaO5S
CAS Number:
62625-32-5
Molecular Weight:
720.00
NACRES:
NA.47
PubChem Substance ID:
24891573
UNSPSC Code:
12171500
EC Number:
263-657-4
MDL number:
MFCD00148898

Key Documents

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Product Name

Bromocresol Green sodium salt, crystalline

InChI key

HEFSGAHJDGZCHA-UHFFFAOYSA-M

InChI

1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1

SMILES string

[Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

agency

suitable for SM 2310
suitable for SM 2320

form

crystalline

visual transition interval

3.8-5.4, yellow to blue

mp

230 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

λmax

616 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Bromocresol Green sodium salt has been used as a pH indicator for glutamate decarboxylase activity assay in Listeria monocytogenes strains.

Biochem/physiol Actions

Bromocresol Green is mainly used for measuring serum albumin. In presence of acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 4.0) to blue color (5.4).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT1985
SHBS4109
SHBR7149
SHBQ9224
SHBP7951

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Remington: The Science and Practice of Pharmacy (2006)
Screening of glutamate decarboxylase activity and bile salt resistance of human asymptomatic carriage, clinical, food, and environmental isolates of Listeria monocytogenes.
International Journal of Food Microbiology, 93, 87-99 (2004)
Homocysteine as a predictive biomarker in early diagnosis of renal failure susceptibility and prognostic diagnosis for end stages renal disease.
Amin HK, et al.
Renal Failure, 38, 1267-1275 (2016)

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