A7431
Agarose
BioReagent, for molecular biology, Wide range/Standard 3:1
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
About This Item
biological source
algae (marine)
Quality Level
grade
for molecular biology
product line
BioReagent
form
powder
technique(s)
electrophoresis: suitable
EEO
≤0.13
mp
≤90 °C
transition temp
gel point 32.5-38 °C (4% gel)
gel strength
≥1200 g/cm2 (4% gel)
anion traces
sulfate (SO42-): ≤0.15%
foreign activity
DNase, RNase, NICKase, none detected
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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Application
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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