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E4884

Ethylenediaminetetraacetic acid disodium salt dihydrate

Name: Ethylenediaminetetraacetic acid disodium salt dihydrate
Brand: SIAL

ACS reagent, 99.0-101.0%

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₄N₂Na₂O₈ · 2H₂O

CAS Number:

6381-92-6

Molecular Weight:

372.24

Beilstein:

3900609

EC Number:

205-358-3

UNSPSC Code:

12352107

PubChem Substance ID:

24894586

NACRES:

NA.77

grade

ACS reagent

Quality Level

200

Agency

suitable for SM 4500 - NH3

Assay

99.0-101.0%

form

solid

reaction suitability

reagent type: chelator

impurities

≤0.005% Insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

pH

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

solubility

H₂O: 100 g/L at 20 °C

cation traces

Fe: ≤0.01%
heavy metals: ≤0.005% (by ICP-OES)

application(s)

microbiology

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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Biological Buffers

Chelating & Reducing Agents for Research

General description

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Other Notes

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comparable product

Product No.

Description

Pricing

Signal Word

Warning

Hazard Statements

H332,H373

Precautionary Statements

P260 - P271 - P304 + P340 + P312 - P314 - P501

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Lot/Batch Number

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