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95039

Millipore

Tryptone enzymatic digest from casein

for biotechnological purposes

Synonym(s):

Peptone from casein, Peptone from casein, tryptic digest

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About This Item

CAS Number:
91079-40-2
EC Number:
293-428-4
UNSPSC Code:
41106212
NACRES:
NA.85

biological source

bovine milk

Quality Level

200

grade

for biotechnological purposes

form

powder

composition

amino-nitrogen, ≥3.5%
total nitrogen (N), ≥12%

ign. residue

≤17.0%

loss

≤6.0% loss on drying

pH

6.0-7.5 (25 °C, 2% in H2O)

solubility

H2O: 2%, clear, faintly to slightly yellow

application(s)

food and beverages
microbiology

storage temp.

15-25°C

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General description

Tryptone is a general-purpose peptone produced by the digestion of casein with the protease trypsin. It is used for the cultivation of most bacteria, fungi, and some protozoa. Casein is the main protein of milk, and a rich source of nitrogenous nutrients (in the amino acids). This peptone is recommended for culture media in analytical microbiology and in industrial fermentation.

Application

Tryptone from enzymatic casein digest can be developed for use in molecular genetic media such as – NZCYM, NZYM and NZM broth. The enriched NZ media can be used to: -
  • Grow recombinant strains of E. coli
  • Propagate theta bacteriophages

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Ohana Y A Costa et al.
Microorganisms, 8(2) (2020-02-16)
The phylum Acidobacteria is widely distributed in soils, but few representatives have been cultured. In general, Acidobacteria are oligotrophs and exhibit slow growth under laboratory conditions. We sequenced the genome of Granulicella sp. WH15, a strain obtained from decaying wood

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Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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