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93735

Millipore

TSN Agar

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.5 g/L
casein peptone, 15 g/L
ferric citrate, 0.5 g/L
neomycin sulfate, 0.05 g/L
polymyxin B sulfate, 0.02 g/L
sodium sulfite, 1 g/L
yeast extract, 10 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Clostridium spp.

Related Categories

Application

Highly selective medium for the detection and enumeration of Clostridium perfringens in food and other material.

Preparation Note

Dissolve 40 g in 1 litre distilled water and autoclave gently at 121°C for 10 minutes. The medium should be used on the day of preparation.

Other Notes

Enumeration of Clostridium perfringens; Enumeration of sulphite reducing clostridia occurring in foods

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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R S MARSHALL et al.
Applied microbiology, 13, 559-563 (1965-07-01)
A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of
D.A.A. Mossel
Journal of the Science of Food and Agriculture, 10, 662-662 (1959)

Articles

Detection, Identification and Differentiation of Clostridium perfringens

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service