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Tris-EDTA buffer solution

BioUltra, for molecular biology, pH 7.4

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Synonym(s):
TE buffer solution
MDL number:
UNSPSC Code:
41105319
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
ND.02

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

7.4±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.01

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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Application

Tris-EDTA buffer solution has been used for the dilution of DNA samples. It has been used for the elution of purified vector.

Physical form

10mM Trizma® base; 1mM EDTA

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications.
Torella JP et al.
Nature Protocols, 9, 2075-2075 (2014)
Sin Hang Lee et al.
International journal of environmental research and public health, 16(10) (2019-05-30)
Lyme disease, initially described as Lyme arthritis, was reported before nucleic-acid based detection technologies were available. The most widely used diagnostic tests for Lyme disease are based on the serologic detection of antibodies produced against antigens derived from a single
The effect of COMT Val158Met and DRD2 C957T polymorphisms on executive function and the impact of early life stress.
Klaus K et al.
Brain and Behavior, 7, e00695-e00695 (2017)
Ying Chen et al.
Applied and environmental microbiology, 74(19), 6132-6137 (2008-08-19)
The relative expression levels of six botulinum neurotoxin cluster genes in a group II Clostridium botulinum type E strain grown at 10 or 30 degrees C were investigated using quantitative real-time reverse transcription-PCR. An enzyme-linked immunosorbent assay was used to
Kyla R Rodgers et al.
Scientific reports, 10(1), 7047-7047 (2020-04-29)
Acute inflammation is a key feature of innate immunity that initiates clearance and repair in infected or damaged tissues. Alternatively, chronic inflammation is implicated in numerous disease processes. The contribution of neuroinflammation to the pathogenesis of neurological conditions, including infection

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