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Merck
CN

92322

Rappaport Vassiliadis medium (base), modified, semi-solid

suitable for microbiology, NutriSelect® Plus

Synonym(s):

MSRV medium (base), Modified Semisolid Rappaport Vassiliadis Medium (base)

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606

Key Documents

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Product Name

Rappaport Vassiliadis medium (base), modified, semi-solid, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 2.7 g/L
casein peptone, 4.59 g/L
magnesium chloride, 10.93 g/L
malachite green, 0.037 g/L
mono-potassium phosphate, 1.47 g/L
sodium chloride, 7.34 g/L
tryptose, 4.59 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.2±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation
microbiology

storage temp.

2-8°C

suitability

selective for Salmonella spp.

Quality Level

200

Application

The modified Semi-Solid Rappaport Vassiliadis medium is formulated according De Smedt and et al. for enrichment of Salmonellae.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 31.6 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add 20 mg/L of Novobiocin (Cat. No. 74675). Mix well and pour into sterile petri plates. Keep plates in a fresh place to settle the gel (1 hour minimum) and handle with care because the medium is only semi-solid.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCL4423
BCCJ9807
BCCG8917
BCCH9540
BCCF0232

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Articles

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

沙门氏菌分离和鉴定培养基

沙门氏菌污染是全球食源性疾病的第二大成因。控制沙门氏菌的暴发是食品监管机构、餐馆和整个食品工业的一项重要任务。沙门氏菌家族包括2300多种血清型的细菌，但其中有两种类型，即肠炎沙门氏菌和鼠伤寒沙门氏杆菌，约占所有人类感染的一半。大多数沙门氏菌的暴发可以追溯到乳制品、家禽和肉制品，但沙门氏菌几乎可以在任何食物上生长。鸡、鸡蛋及其衍生产品的风险特别高。

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