861405
Hoechst 33258
Synonym(s):
2′-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride hydrate, bisBenzimide
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About This Item
Empirical Formula (Hill Notation):
C25H24N6O · 3HCl · xH2O
CAS Number:
Molecular Weight:
533.88 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
Recommended Products
Assay
≥98% (TLC)
form
powder
mp
>300 °C (lit.)
λmax
343 nm
SMILES string
Cl[H].Cl[H].Cl[H].[H]O[H].CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5[nH]c(nc5c4)-c6ccc(O)cc6
Other Notes
This product has been replaced by B2883-Sigma-Aldrich | bisBenzimide H 33258 ≥98% (HPLC and TLC)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Shawn N Whitehead et al.
PloS one, 6(6), e20808-e20808 (2011-06-21)
Gangliosides, a member of the glycosphingolipid family, are heterogeneously expressed in biological membranes and are particularly enriched within the central nervous system. Gangliosides consist of mono- or poly-sialylated oligosaccharide chains of variable lengths attached to a ceramide unit and are
Stud. Biophys., 125, 121-121 (1988)
Hélène Labit et al.
Nucleic acids research, 36(17), 5623-5634 (2008-09-04)
Replication origins in Xenopus egg extracts are located at apparently random sequences but are activated in clusters that fire at different times during S phase under the control of ATR/ATM kinases. We investigated whether chromosomal domains and single sequences replicate
Norman Zielke et al.
Genes & development, 22(12), 1690-1703 (2008-06-19)
Endoreplicating cells undergo multiple rounds of DNA replication leading to polyploidy or polyteny. Oscillation of Cyclin E (CycE)-dependent kinase activity is the main driving force in Drosophila endocycles. High levels of CycE-Cdk2 activity trigger S phase, while down-regulation of CycE-Cdk2
G A Daxhelet et al.
Analytical biochemistry, 179(2), 401-403 (1989-06-01)
The increase in fluorescence, upon interaction with several fluorescent dyes was found to depend on the base composition of DNA. 4',6-Diamidino-2-phenylindole-2 HCl and Hoechst 33258 which bind to AT base pairs show a logarithmic relation. This relation is linear when
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