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Sigma-Aldrich

Wright stain

certified by the Biological Stain Commission

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Synonym(s):
Eosin Methylene blue according to Wright
CAS Number:
68988-92-1
MDL number:
MFCD00082143
UNSPSC Code:
12171500
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

Assay

≤4% (HPLC)

form

powder

concentration

≤4% (HPLC)

solubility

methanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1

InChI key

AXIKDPDWFVPGOD-UHFFFAOYSA-O

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General description

Wright Stain, a modified Romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B. Upon staining blood films with Wright Stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.

Application

  • Wright Stain is intended for use in staining peripheral blood smears and bone marrow smears.
  • It has been used to study the cytogenetics of human hematopoietic cells.
  • It has also been employed in the karyotyping of human embryonic stem cells.
  • It is also used for G-banding and helps in the analysis of poorly spread metaphases.
Reported to be superior to May-Grunwald Stain in blood staining.

Features and Benefits

  • The purified dyes in Wright Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.
  • Reported to be superior to May-Grunwald Stain in blood staining.
  • Certified by the Biological Stain Commission.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Histopathologic Technic and Practical Histochemistry, 390-390 (1954)
Conn?s Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine (10th ed.)
RW Horobin, JA Kiernan
Conn?s Biological Stains (2002)
Rodak BF
Hematology: Clinical Principles and Applications (2007)
Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation
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