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861006

Sigma-Aldrich

Eosin B

certified by the Biological Stain Commission, Dye content 90 %

Synonym(s):

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

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About This Item

Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
548-24-3
Molecular Weight:
624.06
Colour Index Number:
45400
Beilstein:
4121964
EC Number:
208-943-1
MDL number:
MFCD00005041
UNSPSC Code:
12171500
PubChem Substance ID:
24888525
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, 90%

solubility

H2O: 1 mg/mL, clear, red

λmax

395 nm
514 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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Application

  • Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
  • It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
  • It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Suitability

Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Restoration of anal sphincter function after myoblast cell therapy in incontinent rats.
Bisson A, et al.
Cell Transplantation, 24, 277-277 (2015)
Theory and Practice of Histological Techniques (2008)
A A Waheed et al.
Journal of biochemical and biophysical methods, 33(3), 187-196 (1996-12-30)
We describe a detailed procedure for estimating a wide range of proteins by the eosin B dye method. At acidic pH, eosin B binds to proteins and absorption of the protein-dye complex at 536-544 nm is proportional to the concentration
Y M Cheng et al.
Neuroscience, 151(4), 1084-1098 (2008-01-19)
We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine
Kristen M Massimine et al.
Antimicrobial agents and chemotherapy, 50(9), 3132-3141 (2006-08-31)
4',5'-Dibromo-2',7'-dinitrofluorescein, a red dye commonly referred to as eosin B, inhibits Toxoplasma gondii in both enzymatic and cell culture studies with a 50% inhibitory concentration (IC(50)) of 180 microM. As a non-active-site inhibitor of the bifunctional T. gondii dihydrofolate reductase-thymidylate
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