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Sigma-Aldrich

Brilliant Cresyl Blue ALD

Certified by the Biological Stain Commission

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Synonym(s):
3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C
Empirical Formula (Hill Notation):
C17H20ClN3O · 0.5ZnCl2
CAS Number:
51716-96-2
Molecular Weight:
385.96
Beilstein:
5690713
EC Number:
279-675-0
MDL number:
MFCD00148901
UNSPSC Code:
12171500
PubChem Substance ID:
24888521
NACRES:
NA.47

Quality Level

200

form

powder or crystals

composition

Dye content, ≥50%

mp

233-236 °C (lit.)

λmax

622 nm

ε (extinction coefficient)

≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L
≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CC\[N+](CC)=C1\C=CC2=Nc3cc(C)c(N)cc3OC2=C1.CC\[N+](CC)=C4\C=CC5=Nc6cc(C)c(N)cc6OC5=C4

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

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Application

Brilliant cresyl blue ALD has been used for the staining of reticulocytes.

Biochem/physiol Actions

Brilliant cresyl blue ALD is responsible for the staining of RNA in reticulocytes.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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