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85766

Millipore

m-Endo Agar LES

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Membrane Endo Agar LES

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
casein hydrolysate, 3.7 g/L
di-potassium hydrogen phosphate, 3.3 g/L
lactose, 9.4 g/L
pararosanilin (fuchsin), 0.8 g/L
peptone from meat, 3.7 g/L
potassium hydrogen phosphate, 1 g/L
sodium chloride, 3.7 g/L
sodium deoxycholate, 0.1 g/L
sodium lauryl sulfate, 0.05 g/L
sodium sulfite, 1.6 g/L
tryptose, 7.5 g/L
yeast extract, 1.2 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2±25 °C

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Escherichia coli
selective and differential for Proteus spp.
selective and differential for Salmonella spp.
selective and differential for coliforms

Related Categories

Application

m-Endo Agar LES can be used for the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration. This method is more reliable and precise than MPN multiple test. It follows the two step membrane filter procedure using Lauryl Sulfate Broth (Cat. No. 17349) as a preliminary enrichment, resulting in higher coliform counts.

Preparation Note

Suspend 51 g in 1 litre of distilled water containing 20 ml of ethanol 96 %. Boil to dissolve completely. Do not autoclave! Cool to 45-50°C and dispense 4 ml amounts into 50-60 mm Petri dishes and allow to solidify. The agar should be at least 1.5mm depth. Do not expose plates to direct sunlight.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

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Ronald M. Atlas
Handbook of Microbiological Media null

Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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