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Sigma-Aldrich

Acid Fuchsin calcium salt

certified by the Biological Stain Commission, Dye content ≥60 %

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Synonym(s):
Acid Magenta, Acid Rubin, Fuchsin S, SS, SS, ST, or SIII, Rubin S
Linear Formula:
C20H17N3O9S3Ca
CAS Number:
123334-10-1
Molecular Weight:
579.64
MDL number:
MFCD00001654
UNSPSC Code:
12171500
PubChem Substance ID:
24888443
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder or crystals

composition

Dye content, ≥60%

λmax

545 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Ca++].Cc1cc(cc(c1N)S([O-])(=O)=O)\C(c2ccc(N)c(c2)S([O-])(=O)=O)=C3\C=CC(=N)C(=C3)S(O)(=O)=O

InChI

1S/C20H19N3O9S3.Ca/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);/q;+2/p-2/b19-11-,21-14?;

InChI key

SVIISRNRODSEHG-SZRYGYPJSA-L

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General description

Acid fuchsine (C.I. 42685) is a sulfonated triaminotriphenylmethane. It is also known as acid magenta. At pH 12-14, acid fuchsine changes from red to colorless.

Application

Acid fuchsine is used as a biological stain in preparations including the histopathological polychrome of Pianese, the Ehrlich-Biondi triacid procedure for blood smears, and the Altmann stain for mitochondria. Various polychrome stains like the Masson polychrome variant; Van Gieson, and Mallory connective tissue stains contain Acid fuchsine.Other applications of acid fuchsine in biology and medicine involve fluorescent staining of latent fingerprints, the tracking of water flow within plants, and adding into an agar growth medium for yeasts.

Suitability

Certified for use in Van Gieson connective tissue stain.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis and testing of biological stains--the Biological Stain Commission Procedures.
D P Penney et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 77(5-6), 237-275 (2003-02-05)

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