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Merck
CN

85463

Simmons Citrate Agar

NutriSelect® Basic, suitable for microbiology

Synonym(s):

Citrate Utilization Test

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606

Key Documents

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Product Name

Simmons Citrate Agar, NutriSelect® Basic, suitable for microbiology

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
ammonium dihydrogen phosphate, 0.2 g/L
bromothymol blue, 0.08 g/L
disodium ammonium phosphate, 0.8 g/L
magnesium sulfate heptahydrate, 0.2 g/L
sodium chloride, 5 g/L
trisodium citrate, 2 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

~7.0

application(s)

agriculture
clinical testing
environmental
food and beverages
water monitoring
microbiology

suitability

nonselective and differential for Escherichia coli
nonselective and differential for Salmonella spp.
nonselective and differential for coliforms
nonselective and differential for molds (General Media)
nonselective and differential for yeasts (General Media)

Quality Level

100

Related Categories

Application

Simmons Citrate Agar can be used for differentiating between fecal coli and members of the aerogenes group based on citrate utilization. It can also be used for the identification of certain fungi and fungi imperfecti. The agar has been used to perform a citrate test.

General description

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It does not allow the growth of Shigella or coliforms.

Other Notes

Selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 23.3 g in 1 litre distilled water and heat to boiling to dissolve. Distribute in tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes. Allow tubes to cool as slants.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCM9069
BCCM1024
BCCL4440
BCCJ8626
BCCJ5322

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R.K. Dart
Microbiology for the Analytical Chemist (1996)
Massilia aurea sp. nov., isolated from drinking
water
Virginia Gallego
International Journal of Systematic and Evolutionary Microbiology (2006)
E Van Kregten et al.
Journal of clinical microbiology, 20(5), 936-941 (1984-11-01)
A culture medium was developed which selectively favored the growth of Klebsiella pneumoniae and Klebsiella oxytoca in Escherichia coli-rich fecal cultures, without the use of antibiotics. The discriminative capacity of this medium was based on the presence of only two
Elmer W. Koneman
Koneman's Color Atlas and Textbook of Diagnostic Microbiology (2006)

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Differentiation and ID Media for Salmonella

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