85257
Bromo(4-sulfobenzoyloxy)bimane tetramethylammonium salt
for fluorescence, ≥90% (HPCE)
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Synonym(s):
4-(Sulfobenzoyloxy)bromobimane tetramethylammonium salt, Thiolyte SB
Empirical Formula (Hill Notation):
C21H26BrN3O7S
Recommended Products
grade
for fluorescence
Assay
≥90% (HPCE)
suitability
in accordance for fluorescence
storage temp.
−20°C
SMILES string
C[N+](C)(C)C.CC1=C(CBr)N2N(C1=O)C(=O)C(C)=C2COC(=O)c3ccc(cc3)S([O-])(=O)=O
Application
fluroescence marker for proteins
Other Notes
Fluorescent, membrane impermeable, water-soluble label for biothiols (proteins & peptides); products are photochemically stable
Regulatory Information
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G L Newton et al.
Analytical biochemistry, 201(1), 30-42 (1992-02-14)
para-Sulfonylbenzoyloxybromobimane (sBBr) was shown to be similar to the fluorescent labeling agent monobromobimane (mBBr) in reacting rapidly and selectively with thiols to produce stable derivatives which are readily separated by HPLC. Chromatography of the sBBr derivative provides a useful means
Determination of biothiols by bromobimane labeling and high-performance liquid chromatography.
G L Newton et al.
Methods in enzymology, 251, 148-166 (1995-01-01)
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