83205
Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)
powder, off-white, ~70 U/mg
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form
powder
specific activity
~70 U/mg
mol wt
Mr ~71000
color
off-white
storage temp.
−20°C
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General description
Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Application
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
It plays an important role in protein, RNA, and DNA synthesis.
Unit Definition
1 U corresponds to the amount of enzyme which will liberate 1 μmol of phosphate from inorganic pyrophosphate per minute at pH 7.2 and 25 °C
Other Notes
Mechanism of action
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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The mechanism of action of yeast inorganic pyrophosphatase.
B S Cooperman
Methods in enzymology, 87, 526-548 (1982-01-01)
Eric E Hernández-Domíguez et al.
Plant science : an international journal of experimental plant biology, 187, 39-48 (2012-03-13)
Phosphorus is an essential element for all living cells, but its availability is often limiting in the soil. Plants have adapted to such limitation and respond to phosphorus deficiency. The soluble inorganic pyrophosphatases (PPase; EC 3.6.1.1) recycle the pyrophosphate produced
Jing-Jing Xiang et al.
Plant physiology, 159(4), 1488-1500 (2012-06-21)
Leaf rolling is an important agronomic trait in rice (Oryza sativa) breeding and moderate leaf rolling maintains the erectness of leaves and minimizes shadowing between leaves, leading to improved photosynthetic efficiency and grain yields. Although a few rolled-leaf mutants have
J Jämsen et al.
Biochemistry. Biokhimiia, 77(2), 165-170 (2012-02-22)
We earlier described CBS-pyrophosphatase of Moorella thermoacetica (mtCBS-PPase) as a novel phosphohydrolase that acquired a pair of nucleotide-binding CBS domains during evolution, thus endowing the protein with the capacity to be allosterically regulated by adenine nucleotides (Jämsen, J., Tuominen, H.
H Y Lin et al.
Diabetologia, 55(10), 2703-2712 (2012-07-20)
The transcription factor, forkhead box (FOX)O1, is involved in fatty acid-induced apoptosis in pancreatic beta cells, but the precise mechanism is poorly understood. We aimed to identify which direct downstream targets of FOXO1 are involved in palmitate-induced apoptosis in the
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