Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(1)

Key Documents

View All Documentation

Safety Information

77515

Sigma-Aldrich

Methylene Blue solution

Synonym(s):

Ehrlich’s reagent III, Loeffler’s Methylene Blue

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Beilstein:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12171500
PubChem Substance ID:
329767738
NACRES:
NA.47
To inquire about this product 77515, please contact your local Merck office or dealer. Contact Technical Service

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

suitable for microscopy
Corynebacterium spp.
bacteria
mycobacteria
spore forming microorganisms

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
567532U567531U567502U
Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm

567501U

Discovery ® HS F5 HPLC 色谱柱

Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 15 cm × 4 mm

567532U

Discovery ® HS F5 HPLC 色谱柱

Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 10 cm × 4 mm

567531U

Discovery ® HS F5 HPLC 色谱柱

Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 10 cm × 2.1 mm

567502U

Discovery ® HS F5 HPLC 色谱柱

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

agency

suitable for USP L43

agency

suitable for USP L43

agency

suitable for USP L43

agency

suitable for USP L43

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

Application

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

Components

Composition:
methylene blue 0.3 g
ethanol 30 ml
water 100 ml

Other Notes

This product has been replaced by 03978-SIGMA | Methylene Blue solution for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

replaced by

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

113.0 °F

Flash Point(C)

45 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCN0658
    BCCL3727
    BCCM2781
    BCCL6696
    BCCK1973

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Jonel Steffen et al.
    The British journal of ophthalmology, 98(1), 13-15 (2013-10-26)
    To evaluate the diagnostic accuracy of methylene blue used as a non-invasive in vivo stain to detect ocular surface squamous neoplasia (OSSN). A test validation study was performed according to Standards for the reporting of diagnostic accuracy studies (STARD) guidelines
    Xiaojuan Bai et al.
    Langmuir : the ACS journal of surfaces and colloids, 29(9), 3097-3105 (2013-02-20)
    ZnO1-x/graphene hybrid photocatalyst was prepared via a facile in-situ reduction of graphene oxide and ZnO1-x surface defect oxide. The hybrid photocatlayst showed enhanced photocatalytic activity for the photodegradation of methylene blue. The photocorrosion of ZnO1-x was successfully inhibited by graphene
    Q R J G Tummers et al.
    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 40(7), 850-858 (2014-05-28)
    Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of
    Nicolaas Lucius Gerlach et al.
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 72(4), 666-671 (2014-02-01)
    To establish the accuracy of cone-beam computed tomographic (CBCT) views in determining the position and diameter of the mandibular canal. Two freshly frozen cadaver heads, 1 dentate and 1 edentate, were used to acquire CBCT scans. Measurements on cross-sectional CBCT
    Bram Vivijs et al.
    Applied and environmental microbiology, 80(19), 6054-6061 (2014-07-27)
    Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service