Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

77187

Millipore

Buffered Peptone Water

NutriSelect® Plus, powder, pack of 500g or 25kg, pack of 500 g or 25 kg, non-sterile

Synonym(s):

BPW, Buffered Peptone Water, Tryptone Phosphate Water

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

Agency

according to GB 4789.30-2016
according to GB 4789.40-2016
according to ISO 21528-1:2017
according to ISO 22964:2017

Quality Level

200

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

disodium hydrogen phosphate, 3.5 g/L
peptone, 10 g/L
potassium dihydrogen phosphate, 1.5 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Plus

packaging

pack of 500 g or 25 kg
pack of 500g or 25kg

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

color

faintly beige Powder, Homogeneous, free-flowing powder
clear faintly brownish-yellow Prepared media, without any precipitate

final pH

7.2±0.2 (25 °C)

application(s)

environmental
food and beverages
microbiology
pathogen testing
sample preparation

suitability

Providencia spp.
nonselective for Citrobacter spp.
nonselective for Cronobacter spp.
nonselective for Escherichia coli
nonselective for Klebsiella spp.
nonselective for Proteus spp.
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for Yersinia spp.
nonselective for bacteria (General Media)
nonselective for coliforms
nonselective for enterobacteriaceae

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity. It is also useful for the non-selective pre-enrichment of media for bacteria, in particular pathogenic members of the Enterobacteriaceae (sub-lethal damaged), from food and other material.

Application

Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

Preparation Note

Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Storage and Stability

  • Store prepared media below 8°C, protected from direct light.
  • Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.

Other Notes

Studies with salmonellae

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCF6775
BCCD8525
BCCC9736
BCCB8496
BCBZ0769

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

H J Beckers et al.
The Journal of applied bacteriology, 62(2), 97-104 (1987-02-01)
Studies have been carried out in which growth patterns of a Salmonella sp. and competing micro-organisms, especially other Enterobacteriaceae, were followed during pre-enrichment in buffered peptone water (BPw) and subsequent selective enrichment in tetrathionate broth (TBB). Pre-enrichment cultures were inoculated
Dongyou Liu
Molecular Detection of Foodborne Pathogens , 169-169 (2009)
Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts.
Mayachiew, Pornpimon, and Sakamon Devahastin.
LWT--Food Science and Technology, 41, 1153-1159 (2008)
Dario De Medici et al.
Applied and environmental microbiology, 69(6), 3456-3461 (2003-06-06)
The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis

Articles

Chronobacter spp. Classic and Detection Methods

Cronobacter spp. are frequently found on vegetables, meat, fermented bread, dairy and in baby food. Consumption of contaminated powdered infant formula milk can result in sepsis, infant meningitis and necrotizing enterocolitis.

Pseudomonas Media and Tests

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service