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75315

Millipore

OF Test Nutrient Agar

NutriSelect® Basic, suitable for microbiology

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Synonym(s):
Oxidative Fermentative Agar acc. to Hugh a. Leifson
UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 2.5 g/L
bromothymol blue, 0.08 g/L
casein peptone (pancreatic), 2 g/L
dipotassium hydrogen phosphate, 0.3 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture
clinical testing
environmental
food and beverages
microbiology

suitability

Alcaligenes spp.
Chromobacterium spp.
Moraxella spp.
Pasteurella spp.
Acinetobacter spp.
Aeromonas spp.
flavobacteria
nonselective and differential for Bordetella spp.
nonselective and differential for Campylobacter spp.
nonselective and differential for Enterococcus spp.
nonselective and differential for Escherichia coli
nonselective and differential for Pseudomonas spp.
nonselective and differential for Salmonella spp.
nonselective and differential for Shigella spp.
nonselective and differential for Vibrio spp.
nonselective and differential for Yersinia spp.
nonselective and differential for bacteria (General Media)
nonselective and differential for coliforms
nonselective and differential for enterobacteriaceae

Application

Medium for demonstrating oxidative and fermentative catabolism of carbohydrate acc. to Hugh and Leifson (1953). In particular for the differentiation and classification of gram-negative intestinal bacteria.

Preparation Note

Dissolve 9.88 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Let cool to approx. 50°C and add 100 ml of sterile filtered 10% D(+)-glucose (Cat. No. 49140), lactose (Cat. No. 61340), sucrose (Cat. No. 84100) or other carbohydrate.

Other Notes

Studies on coagulase-positive staphylococci from bovine milk

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J A Erasmus
The Onderstepoort journal of veterinary research, 52(1), 25-29 (1985-03-01)
When conventional techniques were applied to 84 isolates of Staphylococcus aureus from milk samples, it was found that they were all catalase and phosphatase positive and oxidase negative. They all fermented glucose within 24 h and mannitol within 24-48 h
The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria.
R HUGH et al.
Journal of bacteriology, 66(1), 24-26 (1953-07-01)

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