70192
Mueller Hinton Broth
suitable for microbiology, NutriSelect® Plus
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Synonym(s):
MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media
UNSPSC Code:
41171504
NACRES:
NA.74
Recommended Products
sterility
non-sterile
Quality Level
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
composition
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L
manufacturer/tradename
NutriSelect® Plus
storage condition
protect from light
technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
final pH
7.4±0.2 (25 °C)
application(s)
clinical testing
food and beverages
microbiology
storage temp.
15-25°C
suitability
nonselective for bacteria (General Media)
Related Categories
General description
Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.
Application
Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus
Preparation Note
Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J.H. Muller et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 48, 330-330 (1941)
Dennis Wilkens Juhl et al.
Frontiers in cellular and infection microbiology, 10, 526459-526459 (2020-10-27)
Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides
Tracy Lackraj et al.
Journal of bacteriology, 202(11) (2020-04-02)
Dps, a DNA-binding protein from starved cells in Escherichia coli, is part of the bacterial defense system that protects DNA against various cellular stresses. Our lab previously demonstrated that a novel antimicrobial peptide, WRWYCR, enhances acid-induced killing of enterohemorrhagic Escherichia
Branko Jovčić et al.
Antibiotics (Basel, Switzerland), 9(12) (2020-12-17)
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven
Victoria L Barlow et al.
Scientific reports, 10(1), 20464-20464 (2020-11-26)
Acinetobacter baumannii is a prevalent pathogen that can rapidly acquire resistance to antibiotics. Indeed, multidrug-resistant A. baumannii is a major cause of hospital-acquired infections and has been recognised by the World Health Organization as one of the most threatening bacteria
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