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70188

Millipore

Violet Red Bile Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
VRB Agar, VRBL-Agar
UNSPSC Code:
41171606
NACRES:
NA.74

Agency

according to ISO 4832:2006

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L
bile salts, 1.5 g/L
crystal violet, 0.002 g/L
lactose, 10.0 g/L
neutral red, 0.03 g/L
peptone, 7.0 g/L
sodium chloride, 5.0 g/L
yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for coliforms

Related Categories

Application

A selective medium for the detection and enumeration of coliform organisms, especially the Coli-aerogenes-group in water, milk and other dairy products.

Preparation Note

Suspend 38.5 g in 1 litre of distilled water and let it sock for 5 minutes. Bring to boil and distribute into plates or tubes. Sterilization at 121°C for 15 minutes is optional, but if the medium is to be used on the same day of preparation it needs to be sterilized. But through sterilization may the medium is going to be turbid. No sterilization improves the recovery of the stressed microorganisms.

Other Notes

Recommended by the American Public Health Association (APHA); Studies on coliform bacteria

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G. Suhren
Milchwiss., 45, 139-139 (1990)
J. Klose
Susswaren, 14, 778-778 (1968)
Mater H Mahnashi et al.
Journal of ethnopharmacology, 273, 113976-113976 (2021-03-02)
Members of Orchidaceae family has a long history in herbal and Chinese medicines. Members of this family are most commonly famous in the management of inflammation and analgesia in folk medicine. Habenaria digitata, an unexplored specie of Orchidaceae is found
J.G. Davis
Milk Testing, 131-131 (1951)
F.I.L./I.D.F., Milchwiss., 29, 602-602 (1974)
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Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

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